Sporulation and enterotoxin production by Clostridium perfringens type A at 37 and 43 degrees C
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چکیده
منابع مشابه
Sporulation and enterotoxin production by mutants of Clostridium perfringens.
The ability of Clostridium perfringens type A to produce an enterotoxin active in human food poisoning has been shown to be directly related to the ability of the organism to sporulate. Enterotoxin was produced only in a sporulation medium and not in a growth medium in which sporulation was repressed. Mutants with an altered ability to sporulate were isolated from an sp(+) ent(+) strain either ...
متن کاملCodY Promotes Sporulation and Enterotoxin Production by Clostridium perfringens Type A Strain SM101.
Clostridium perfringens type D strains cause enterotoxemia and enteritis in livestock via epsilon toxin production. In type D strain CN3718, CodY was previously shown to increase the level of epsilon toxin production and repress sporulation. C. perfringens type A strains producing C. perfringens enterotoxin (CPE) cause human food poisoning and antibiotic-associated diarrhea. Sporulation is crit...
متن کاملClostridium perfringens Sporulation and Enterotoxin Synthesis
5 Running title: Role of SigF and SigG in sporulation and CPE synthesis 6 * To Whom Correspondence Should be Addressed: 7 Bruce A. McClane 8 Department of Microbiology and Molecular Genetics 9 447 Bridgeside Point II Building 10 450 Technology Drive 11 University of Pittsburgh School of Medicine 12 Pittsburgh, PA 15219 13 Phone: 412-648-9022 14 Fax: 412-624-1401 15 Email: [email protected] 16 Copy...
متن کاملClostridium perfringens type A: in vitro system for sporulation and enterotoxin synthesis.
Polysomes were isolated from an enterotoxigenic strain of Clostridium perfringens during vegetative growth and at 1-h intervals after transfer into Duncan-Strong sporulation medium. During vegetative growth, about 67% of the ribosomes were in polysomal complexes. This proportion decreased to about 20% during the first 2 h in sporulation medium and then gradually increased to a maximum of 45% at...
متن کاملPurification and biochemical properties of Clostridium perfringens type A enterotoxin.
The sporulation-specific enterotoxin of Clostridium perfringens type A, which is the toxin active in human food poisoning, has been purified from extracts of sporulating cells. Highly purified enterotoxin was obtained by treatment of crude cell extract with ribonuclease for 30 min, followed by sequential chromatography on Sephadex G-100, Cellex T cellulose, and hydroxylapatite. Recovery was 65 ...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1992
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.58.4.1411-1414.1992